2018年深圳市学校结核病临床分离株的MIRU-VNTR基因分型及传播特点分析

doi:10.19871/j.cnki.xfcrbzz.2021.03.011

新发传染病电子杂志 ›› 2021, Vol. 6 ›› Issue (3): 215-219.doi: 10.19871/j.cnki.xfcrbzz.2021.03.011

2018年深圳市学校结核病临床分离株的MIRU-VNTR基因分型及传播特点分析

谢丽, 杨争, 尤信信, 符娇兰, 王峰, 杨慧

深圳市慢性病防治中心中心实验室,广东深圳 518020

收稿日期:2021-03-31 出版日期:2021-08-31 发布日期:2021-10-11
通讯作者: 杨慧,Email：yh2009cn@qq.com
基金资助:
1.广东省自然科学基金（2020A1515011086）; 2.深圳市卫生系列科研项目资助（SZBC2018009）

MIRU-VNTR genotyping characteristics and transmission analysis of clinical isolates of tuberculosis in Shenzhen schools in 2018

Xie Li, Yang Zheng, You Xinxin, Fu Jiaolan, Wang Feng, Yang Hui

Shenzhen Center For Chronic Disease Control Central Laboratory, Guangdong Shenzhen 518020, China

Received:2021-03-31 Online:2021-08-31 Published:2021-10-11

摘要/Abstract

摘要： 目的应用可变数目串联重复序列（variable- number tandem repeat,VNTR）基因分型技术对学校结核病的流行情况进行监测,了解2018年深圳市学校结核病临床分离株的基因多态性及近期传播情况。方法收集深圳市慢性病防治机构及定点医院学校结核病患者培养阳性菌株,提取基因组DNA,采用聚合酶链反应（polymerase chain reaction,PCR）对12个MIRU-VNTRs位点进行检测。检测结果采用MIRU-VNTRs在线分析软件和MEGA7.6软件进行基因分型、聚类分析和进化树图绘制。结果 2018年深圳市慢性病防治中心共收集63例学校结核分枝杆菌培养阳性菌株,其中有来自学生患者的菌株52例（82.5%）,教职工患者菌株11例(17.5%)。学生结核病主要发生在高中/职高（44.23%）和大学（40.38%）,而教职工结核病主要发生在幼儿园（54.5%）。63株结核分枝杆菌分为59个基因型,包括56个独特基因型,3个基因簇;其中北京基因型菌株43株（68.3%）,非北京基因型17株（27.0%）。在3所学校内均发现2例患者感染结核分枝杆菌株的案例,流行病学调查结果分别判断为独立感染、校内传播和家庭聚集性疫情。结论 2018年深圳市学校结核病临床分离株基因型多样性高,未出现2例以上的聚集性疫情,学校结核疫情的防控状态现状良好,MIRU-VNTRs同源性鉴定技术可应用于聚集性群体结核病分子流行病学调查的常规监测和应急检测。

关键词: 深圳市, 基因分型, 结核分枝杆菌, 近期传播

Abstract: Objective Use variable-number tandem repeat (VNTR) genotyping technology to monitor the prevalence of tuberculosis in schools, and understand the genetic polymorphisms and recent transmission of tuberculosis clinical isolates in Shenzhen schools in 2018. Methods Genomic DNA was extracted from tuberculosis patients from shenzhen center for chronic disease control and designated hospitals, and 12 MIRU-VNTRs sites were detected and analyzed by Polymerase Chain Reaction (PCR).Online analysis software MIRU-VNTRS and MEGA7.6 were used for genotyping cluster analysis and phylogenetic tree mapping. Results A total of 63 tuberculosis culture-positive strains were collected in 2018, including 52 strains (82.5%) from student patients and 11 strains (17.5%) from faculty patients. The incidence of TB among students was 44.23% in high school/vocational school and 40.38% in university, while the incidence of TB among teaching staff was mainly in kindergarten (54.5%). The 63 strains of Mycobacterium tuberculosis were divided into 59 genotypes, including 56 unique genotypes and 3 gene clusters. Among them, 43 strains (68.3%) were Beijing genotype and 17 strains (27.0%) were non-Beijing genotype. In the three schools were found in two patients infected with strains of TB cases, and the results of the tuberculosis epidemiological investigation were determined to be independent infection, intra-school transmission and family cluster epidemic. Conclusion In 2018, the genotype diversity of tuberculosis strains in Shenzhen schools was high, and there were no more than 2 cases of clustered epidemics. The status of prevention and control of tuberculosis epidemics in schools is good. The MIRU-VNTRS homology identification technology can be used for routine monitoring and emergency detection of molecular epidemiological investigation of clustered tuberculosis in population.

Key words: Shenzhen city, Genotyping, Mycobacterium Tuberculosis, Recent transmission

谢丽, 杨争, 尤信信, 符娇兰, 王峰, 杨慧. 2018年深圳市学校结核病临床分离株的MIRU-VNTR基因分型及传播特点分析[J]. 新发传染病电子杂志, 2021, 6(3): 215-219.

Xie Li, Yang Zheng, You Xinxin, Fu Jiaolan, Wang Feng, Yang Hui. MIRU-VNTR genotyping characteristics and transmission analysis of clinical isolates of tuberculosis in Shenzhen schools in 2018[J]. Electronic Journal of Emerging Infectious Diseases, 2021, 6(3): 215-219.

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2018年深圳市学校结核病临床分离株的MIRU-VNTR基因分型及传播特点分析

MIRU-VNTR genotyping characteristics and transmission analysis of clinical isolates of tuberculosis in Shenzhen schools in 2018

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