基质辅助激光解吸电离飞行时间质谱在幽门螺杆菌检测中的应用

doi:10.19871/j.cnki.xfcrbzz.2021.03.010

新发传染病电子杂志 ›› 2021, Vol. 6 ›› Issue (3): 210-214.doi: 10.19871/j.cnki.xfcrbzz.2021.03.010

基质辅助激光解吸电离飞行时间质谱在幽门螺杆菌检测中的应用

张淑贞, 李雪宏, 黄海燕, 陈海婷

深圳市大鹏新区葵涌人民医院检验科,广东深圳 518119

收稿日期:2021-03-31 出版日期:2021-08-31 发布日期:2021-10-11
通讯作者: 李雪宏,Email：lihongsz@163.com
基金资助:
1.深圳市大鹏新区医疗健康集团2019年度科研项目(2019JTLCYJ003),2.深圳市医疗卫生“三名工程”项目（SZSM201510050）

Detection of Helicobacter pylori by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Zhang Shuzhen, Li Xuehong, Huang Haiyan, Chen Haiting

Clinical Laboratory Department of Shenzhen Dapeng New District Kuichong People's Hospital, Guangdong Shenzhen 518119, China

Received:2021-03-31 Online:2021-08-31 Published:2021-10-11

摘要/Abstract

摘要： 目的建立并评价基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）对幽门螺杆菌（Hp）的鉴定能力及Hp耐药性菌株对检测结果的影响。方法 MALDI-TOF MS自建库中的Hp来源于样本库中经传统方法鉴定和药物敏感试验并已进行全基因测序确认的菌株,随机取其中对药物全敏感的Hp菌株10株,用MALDI-TOF MS进行测定并采集有效质谱建立Hp鉴定超级质谱,然后用临床新收集的胃黏膜活检样本经培养鉴定、药物敏感试验和全基因测序确认获得的201株Hp菌株进行验证和评价。结果 201株Hp菌株经MALDI-TOF MS检测196株阳性,鉴定阳性率97.5%（196/201）;按药物敏感试验分组结果,敏感株33株全部阳性,鉴定阳性率100%(33/33),耐药株168株有163株阳性,鉴定阳性率97.0%（163/168）,两组比较差异无统计学意义（P>0.05）。结论 MALDI-TOF MS鉴定Hp结果准确,缩短了培养和鉴定的时间,有较好的临床应用价值;Hp耐药性对鉴定结果的准确性无显著性影响。

关键词: 基质辅助激光解吸电离飞行时间质谱, 幽门螺杆菌, 耐药性

Abstract: Objective Establish the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)'s ability of identifying Helicobacter pylori (Hp) and evaluate the effect of Hp-resistant strains on the results. Methods The Hp strains which were verified by traditional methods, drug resistance tests and confirmed by full gene sequencing were used for building MALDI-TOF MS library. 10 drug-sensitive Hp strains from the library were randomly collected to establish Hp identification Super Spectrum. Then, 201 Hp strains obtained from newly collected gastric mucosal biopsy samples were tested and evaluated by the MALDI-TOF MS. They were verified by culture identification, drug resistance test, and complete gene sequencing as well. Results The 201 Hp strains were tested by the MALDI-TOF MS. 196 strains of them were positive. The positive rate of MALDI-TOF MS was 97.5% (196/201). 33 strains of them were drug sensitive, which were all positive and the positive rate was 100% (33/33); 168 stains were drug-resistant. 163 strains of them were positive and the positive rate was 97.0% (163/168). There was no statistically significant difference between the two groups (P>0.05). Conclusion The identification of Hp by MALDI-TOF MS is accurate, which can greatly shorten the time of culture and shows a good clinical application value. The Hp-resistant strains have no significant effect on the identification accuracy.

Key words: Matrix-assisted laser desorption ionization time of flight mass spectrometry, Helicobacter pylori, Drug resistanc

张淑贞, 李雪宏, 黄海燕, 陈海婷. 基质辅助激光解吸电离飞行时间质谱在幽门螺杆菌检测中的应用[J]. 新发传染病电子杂志, 2021, 6(3): 210-214.

Zhang Shuzhen, Li Xuehong, Huang Haiyan, Chen Haiting. Detection of Helicobacter pylori by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry[J]. Electronic Journal of Emerging Infectious Diseases, 2021, 6(3): 210-214.

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基质辅助激光解吸电离飞行时间质谱在幽门螺杆菌检测中的应用

Detection of Helicobacter pylori by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

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