A case of comprehensive analysis and diagnosis of mycobacterium tuberculosis complicated with multi-pathogen pneumonia by pathogens targeted next-generation sequencing

doi:10.19871/j.cnki.xfcrbzz.2024.03.016

Abstract

Abstract: Mycobacterium tuberculosis is the primary causative agent of human tuberculosis. Conventional detection methods for Mycobacterium tuberculosis, such as acid-fast staining and Roche culture method and Interferon gamma release assay (IGRA) , are time-consuming and have low sensitivity in diagnosing tuberculosis, often leading to clinical misdiagnosis, missed diagnosis, and treatment delays. In recent years, the integration of targeted Next-generation sequencing (tNGS) technology with Multiplex polymerase chain reaction (MPCR) amplification and high-throughput sequencing has gained increasing attention in the field of clinical testing due to its advantages of rapidity,ultra-broad spectrum, high sensitivity and high detection rate. It has gradually emerged as an important complementary approach for identifying Mycobacterium tuberculosis .This paper reported a 93-year-old male patient with severe pneumonia in the Longhua District People's Hospital of Shenzhen. The clinical manifestation, imaging examination, pathogen detection and treatment process were analyzed. The patient was finally diagnosed as Mycobacterium tuberculosis complicated with multi-pathogen infection by tNGS. tNGS showed potential value in earlier diagnosis, mixed infection identification, surveillance and therapeutic efficacy evaluation. This not only enhances the clinical differential diagnosis of Mycobacterium tuberculosis but also advances the understanding among healthcare professionals regarding the application of tNGS in pathogen identification for infectious diseases.

Key words: Lung infection, Severe pneumonia, Mycobacterium tuberculosis, Targeted Next-generation sequencing

CLC Number:

Liu Tengyingzi, Liu Nanxi, Zhang Xiaomin, Niu Dandan, Guo Yongchao, Wang Dan, Lin Dachuan, Li Tong, Huang Manyu, Zhang Yingluan, Zhang Zhen, Shi Xiaolu, Feng Tiejian. A case of comprehensive analysis and diagnosis of mycobacterium tuberculosis complicated with multi-pathogen pneumonia by pathogens targeted next-generation sequencing[J]. Electronic Journal of Emerging Infectious Diseases, 2024, 9(3): 73-77.

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