裂隙有核细胞在百日咳患者中的表达及诊断价值研究进展

doi:10.19871/j.cnki.xfcrbzz.2025.02.015

摘要/Abstract

摘要： 百日咳是常见的传染性疾病,严重危及患者身心健康。百日咳发病机制复杂,尚不甚清楚,考虑与百日咳鲍特菌、百日咳毒素、细胞损伤、持续咳嗽等因素密切相关。百日咳临床发病率较高,危险因素较多,尽早诊断并及时治疗尤为重要。近年来,裂隙有核细胞在百日咳相关研究中受到广泛关注。裂隙有核细胞是淋巴细胞的一种特殊形态,可作为百日咳早期诊断的辅助依据。但裂隙有核细胞单一指标诊断百日咳易造成漏诊与误诊,故联合其他特异性指标和检测方法能够提高临床诊断效能。鉴于此,本文结合国内外文献报道,综述裂隙有核细胞在百日咳患者中的表达水平及在百日咳早期诊断方面的研究进展,为进一步将裂隙有核细胞应用于百日咳的早期快速诊断提供参考。

关键词: 裂隙有核细胞, 百日咳, 诊断价值, 表达水平, 研究进展

Abstract: Pertussis is a common infectious disease that seriously endangers the physical and mental health of patients. Its pathogenesis is complex, closely associated with factors such as Bordetella pertussis, toxins, cellular damage, and persistent cough. Clinically, pertussis has a high incidence and numerous risk factors, making early diagnosis and timely treatment particularly critical. In recent years, cleft nucleated cells—special morphological forms of lymphocytes—have garnered extensive attention in pertussis research. These cells can serve as an auxiliary indicator for early diagnosis due to their specific expression in pertussis patients. However, relying solely on cleft nucleated cells for diagnosis may lead to missed or misdiagnosis. Therefore, combining this marker with other specific indicators and detection methods can enhance clinical diagnostic efficacy. Based on domestic and international literature, this paper reviews the expression levels of cleft nucleated cells in pertussis patients and advances in their application for early diagnosis. The goal is to provide a reference for integrating cleft nucleated cells into rapid early diagnostic protocols for pertussis.

Key words: Cleft nucleated cells, Pertussis, Diagnostic value, Expression level, Research progress

中图分类号:

R516.6

全娜丽, 彭美, 卢远彬, 王慧婷, 林思强. 裂隙有核细胞在百日咳患者中的表达及诊断价值研究进展[J]. 新发传染病电子杂志, 2025, 10(2): 89-93.

Quan Nali, Peng Mei, Lu Yuanbin, Wang Huiting, Lin Siqiang. Advances in expression and diagnostic value of cleft nucleated cells in patients with pertussis[J]. Electronic Journal of Emerging Infectious Diseases, 2025, 10(2): 89-93.

参考文献

[1] 陈一然, 邓继岿. 百日咳家庭聚集发病2例[J/CD]. 新发传染病电子杂志, 2023, 8(2):68-70.
[2] YOUNT KS, HALL JM, CAUTION K, et al.Systemic priming and intranasal booster with a BcfA-adjuvanted acellular pertussis vaccine generates CD4+ IL-17+ nasal tissue resident T cells and reduces B. pertussis nasal colonization[J]. Front Immunol, 2023, 14: 1181876.
[3] PERUSSOLO MC, MOGHARBEL BF, SACAKI CS, et al.Demyelination Lesions Do Not Correlate with Clinical Manifestations by Bordetella pertussis Toxin Concentrations[J]. Life (Basel), 2022, 12(7):962.
[4] 卢峥, 罗飞, 兰礼节, 等. 百日咳患儿外周血涂片发现裂隙淋巴细胞1例[J]. 检验医学与临床, 2020, 17(20):3067-3068.
[5] BADAMI GD, LA MANNA MP, DI CARLO P, et al.Delivery of Mycobacterium tuberculosis epitopes by Bordetella pertussis adenylate cyclase toxoid expands HLA-E-restricted cytotoxic CD8⁺T cells[J]. Front Immunol, 2023, 14: 1289212.
[6] MONTERRUBIO-LóPEZ GP, LLAMAS-MOUROY JL, MAARTINEZ-GóMEZ ÁA, et al. Novel vaccine candidates of Bordetella pertussis identified by reverse vaccinology[J]. Biologicals, 2024, 85: 101740.
[7] AAKARI VR, BARADARAN RAHIMI V, SHAFIEE-NICK R.Low Doses of β-Caryophyllene Reduced Clinical and Paraclinical Parameters of an Autoimmune Animal Model of Multiple Sclerosis: Investigating the Role of CB2 Receptors in Inflammation by Lymphocytes and Microglial[J]. Brain Sci, 2023, 13(7):1092.
[8] CALLENDER M, HARVILL ET.Maternal vaccination: shaping the neonatal response to pertussis[J]. Front Immunol, 2023, 14: 1210580.
[9] CIMOLAI N.Non-primate animal models for pertussis: back to the drawing board?[J]. Appl Microbiol Biotechnol, 2022, 106(4):1383-1398.
[10] HOLUBOVA J, STANEK O, JUHASZ A, et al.The Fim and FhaB adhesins play a crucial role in nasal cavity infection and Bordetella pertussis transmission in a novel mouse catarrhal infection model[J]. PLoS Pathog, 2022, 18(4):e1010402.
[11] CHEN Z, LIU X, ZHANG Y, et al.Evaluation of serum anti-pertussis toxin IgA antibodies for the diagnosis of Bordetella pertussis infection in young children[J]. J Infect Public Health, 2023, 16(8):1167-1173.
[12] GAO J, HUANG L, LUO S, et al.A novel vaccine formulation candidate based on lipooligosaccharides and pertussis toxin against Bordetella pertussis[J]. Front Immunol, 2023, 14: 1124695.
[13] 张倩, 吴丹, 郑徽, 等. 中国2022年百日咳流行病学特征[J]. 中国疫苗和免疫, 2024, 30(1):34-38.
[14] MIGUELENA CHAMORRO B, DE LUCA K, Swaminathan G, et al.Bordetella bronchiseptica and Bordetella pertussis: similarities and differences in infection, immuno-modulation, and vaccine considerations[J]. Clin Microbiol Rev, 2023, 36(3):e00164-22.
[15] 刘娟, 卢秀兰, 朱德胜, 等. 儿童百日咳临床特征及重症百日咳危险因素分析[J]. 中国小儿急救医学, 2022, 29(10):796-802.
[16] SOUMANNA IH, DEWAN KK, LINZ B, et al. Modeling the catarrhal stage of Bordetella pertussis upper respiratory tract infections in mice[J]. Dis Model Mech, 2022, 15(5):dmm049266.
[17] GILLARD J, BLOK BA, GARZA DR, et al.BCG-induced trained immunity enhances acellular pertussis vaccination responses in an explorative randomized clinical trial[J]. NPJ Vaccines, 2022, 7(1):21.
[18] MENG Q, SHI W, LI L, et al.“Cleaved lymphocytes” could be induced by pertussis toxin injection in mice, and are actually not lymphocytes[J]. Clin Infect Dis, 2018, 66(4):639-640.
[19] BRENNER D, GOLKS A, BECKER M, et al.Caspase-cleaved HPK1 induces CD95L-independent activation-induced cell death in T and B lymphocytes[J]. Blood, 2007, 110(12):3968-3977.
[20] ZHANG Z, LI Y, CHEN N, et al.Pertussis toxin-induced inhibition of Wnt/β-catenin signaling in dendritic cells promotes an autoimmune response in experimental autoimmune uveitis[J]. J Neuroinflammation, 2023, 20(1):24.
[21] SHI T, DU S, FAN H, et al.Risk Factors of Exchange Blood Transfusion in Infants With Severe Pertussis[J]. Clin Pediatr(Phila), 2023, 62(10):1245-1253.
[22] ALGHOUMAIM M, ALSAFFAR Z, ALFRAII A, et al.Whole-cell and acellular pertussis vaccine: reflections on efficacy[J]. Med Princ Pract, 2022, 31(4):313-321.
[23] 彭税, 李俊, 邵丽丽, 等. 裂隙淋巴细胞协诊婴儿百日咳1例并文献复习[J]. 中医临床研究, 2020, 12(35):122-124.
[24] WANG P, RAMADAN S, DUBEY P, et al.Development of carbohydrate based next-generation anti-pertussis vaccines[J]. Bioorg Med Chem, 2022, 74: 117066.
[25] 吴少珍, 杨海霞, 黄柏开, 等. 裂隙核细胞及外周血T淋巴细胞亚群在百日咳患儿中的表达探究[J]. 中国医学创新, 2021, 18(23):131-135.
[26] LI A, HE X, WU J, et al.Ultrathin silicon nitride membrane with slit-shaped pores for high-performance separation of circulating tumor cells[J]. Lab Chip, 2022, 22(19):3676-3686.
[27] SIMA ST, DAMTIE D, TESSEMA B, et al.Assessment of lymphocytosis among pertussis patients in Northwest Ethiopia[J]. Vacunas (English Edition), 2024, 25(2):174-180.
[28] GAO Q, XU D, GUAN XY, et al.Development and validation of a diagnostic prediction model for children with pertussis[J]. Sci Rep, 2024, 14(1):17154.
[29] SZWEJSER-ZAWISLAK E, WILK MM, PISZCZEK P, et al.Evaluation of whole-cell and acellular pertussis vaccines in the context of long-term herd immunity[J]. Vaccines (Basel), 2022, 11(1):1.
[30] ERNSTT K.Novel strategies to inhibit pertussis toxin[J]. Toxins, 2022, 14(3):187.
[31] 陈奕颖, 林宏昌. 裂隙淋巴细胞对百日咳的诊断价值[J]. 检验医学与临床, 2020, 17(7):918-920.
[32] CORBIèRE V, LAMBERT EE, RODESCH M, et al. A semi high-throughput whole blood-based flow cytometry assay to detect and monitor Bordetella pertussis-specific Th1, Th2 and Th17 responses[J]. Front Immunol, 2023, 14: 1101366.
[33] 胡杨, 庄严, 吴运芹, 等. 换血治疗在新生儿重症百日咳并高白细胞血症中的应用[J]. 中国当代儿科杂志, 2024, 26(11):1155-1161.
[34] 康利民, 米荣, 王亚娟, 等. 新生儿百日咳61例临床特征分析[J]. 中华新生儿科杂志(中英文), 2025, 40(1):28-33.
[35] 许秀妆, 章金灿. 裂隙淋巴细胞辅助诊断儿童百日咳的临床价值[J]. 中国当代儿科杂志, 2020, 22(9):996-1000.
[36] ORIJE MRP, GARCIA-FOGEDA I, VAN DYCK W, et al.Impact of maternal pertussis antibodies on the infants’ cellular immune responses[J]. Clin Infect Dis, 2022, 75(3):442-452.
[37] 黄广丽, 李军勤, 李杰, 等. 裂隙淋巴细胞联合血细胞检测对百日咳的诊断价值[J]. 浙江医学, 2022, 44(22):2437-2439.
[38] GUO S, ZHU Y, GOU Q, et al.Severe pertussis in infants: a scoping review[J]. Ann Med, 2024, 56(1):2352606.
[39] DIKS AM, VERSTEEGEN P, TEODOSIO C, et al.Age and primary vaccination background influence the plasma cell response to pertussis booster vaccination[J]. Vaccines, 2022, 10(2):136.
[40] JIANG W, WANG X, SU Y, et al.Intranasal immunization with a c-di-GMP-Adjuvanted Acellular pertussis vaccine provides superior immunity against Bordetella pertussis in a mouse model[J]. Front Immunol, 2022, 13: 878832.
[41] ALSWAILMI O, El SHAHIBY S.Role of Peripheral Blood Smear in Rapid and Confirmative Pertussis Diagnosis: A Case Report[J]. Dr Sulaiman Al Habib Med J, 2024, 6(1):30-33.
[42] AGHOUNAIM M, ALSAFFAR Z, ALFRAIJ A, et al.Whole-cell and acellular pertussis vaccine: reflections on efficacy[J]. Med Princ Pract, 2022, 31(4):313-321.