深圳市某花鸟市场鹦鹉热衣原体采样调查与分离株基因组学研究

doi:10.19871/j.cnki.xfcrbzz.2025.03.001

新发传染病电子杂志 ›› 2025, Vol. 10 ›› Issue (3): 1-5.doi: 10.19871/j.cnki.xfcrbzz.2025.03.001

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深圳市某花鸟市场鹦鹉热衣原体采样调查与分离株基因组学研究

崔文凯¹, 吴双², 陈琼城², 雷欣悦¹, 袁力¹, 蔡健光³, 石晓路²

1.南华大学公共卫生学院,湖南衡阳 421001;
2.深圳市疾病预防控制中心病原微生物检验所,广东深圳 518055;
3.南方医科大学公共卫生学院,广东广州 510515

收稿日期:2025-02-18 出版日期:2025-06-30 发布日期:2025-07-24
通讯作者: 石晓路,Email:shixiaolu831@163.com
基金资助:
1.广东省新发传染病应对病原体检测重点实验室（2023B1212010010）;2.广东省自然科学基金一般研究项目（2514050002210）;3.深圳市“医疗卫生三名工程”项目（SZSM202211023）;4.深圳市自然科学基金基础研究项目（JCYJ20240813160806009）

Surveillance of Chlamydia psittaci in a bird market in Shenzhen and genomic characterization of isolated strains

Cui Wenkai¹, Wu Shuang², Chen Qiongcheng², Lei Xinyue¹, Yuan Li¹, Cai Jianguang³, Shi Xiaolu²

1. School of Public Health, University of South China, Hunan Hengyang 421001, China;
2. Department of Pathogen Microbiology Testing, Shenzhen Center for Disease Control and Prevention, Guangdong Shenzhen 518055, China;
3. School of Public Health, Southern Medical University, Guangdong Guangzhou 510515, China

Received:2025-02-18 Online:2025-06-30 Published:2025-07-24

摘要/Abstract

摘要： 目的对深圳地区花鸟市场鹦鹉热衣原体（Chlamydia psittaci,Cps）带菌情况进行筛查,并针对筛查阳性的样本进行全面解析,为鹦鹉热的流行病学研究、疾病防控及公共卫生管理提供科学依据。方法 2023年5月和2024年6月于深圳市某农批市场鹦鹉专卖档口开展2次采样调查工作,对采集的样本进行Cps筛查,阳性样本通过细胞培养的方式分离Cps,对分离出的Cps进行全基因组测序,并进行基因组学分析,探究基因型分布及进化关系。结果共采集110份样本,其中38份样本呈Cps阳性,阳性率为34.5%（38/110）。成功从1只Cps阳性鹦鹉的小肠黏膜中分离出1株Cps。通过全基因组测序获得该分离株完整基因组序列,并鉴定为A基因型。系统进化分析显示,该株Cps与鹦鹉来源的参考株在遗传上关系最为接近,提示其可能具有一定的宿主适应性。结论深圳市某农批市场鹦鹉专卖档口采集的样本中Cps检出率较高,应加强花鸟市场,尤其是鹦鹉档口的卫生管理,强化对Cps的持续监测。

关键词: 鹦鹉热衣原体, 基因组学, 细胞分离, 带菌情况调查分析

Abstract: Objective To screen for the presence of Chlamydia psittaci (Cps) in parrots from bird and flower markets in Shenzhen, and to conduct a comprehensive analysis of the positive samples, providing a scientific basis for epidemiological research, disease prevention and control, and public health management. Method In May 2023 and June 2024, two rounds of investigation and sampling were conducted at a parrot retail stall in a wholesale agricultural market in Shenzhen. The collected samples were screened for Cps, and Cps-positive samples were subjected to isolation through cell culture. Whole-genome sequencing was performed on the isolated Cps strains, followed by genomic analysis to explore genotype distribution and evolutionary relationships. Result A total of 110 samples were collected, of which 38 tested positive for Cps, with a positivity rate of 34.5% (38/110). One Cps strain was successfully isolated from the intestinal mucosa of a Cps-positive parrot. Whole-genome sequencing yielded the complete genome of the isolate, which was identified as genotype A. Phylogenetic analysis revealed that this strain is genetically closest to reference strains derived from parrots, suggesting potential host adaptation. Conclusion A relatively high detection rate of Cps was observed in samples collected from the parrot retail stall in a wholesale agricultural market in Shenzhen. It is recommended to strengthen hygiene management in bird and flower markets, especially at parrot stalls, and to enhance ongoing surveillance of Cps.

Key words: Chlamydia psittaci, Genomics, Cell isolation, Carrier status investigation and analysis

中图分类号:

R47.55

崔文凯, 吴双, 陈琼城, 雷欣悦, 袁力, 蔡健光, 石晓路. 深圳市某花鸟市场鹦鹉热衣原体采样调查与分离株基因组学研究[J]. 新发传染病电子杂志, 2025, 10(3): 1-5.

Cui Wenkai, Wu Shuang, Chen Qiongcheng, Lei Xinyue, Yuan Li, Cai Jianguang, Shi Xiaolu. Surveillance of Chlamydia psittaci in a bird market in Shenzhen and genomic characterization of isolated strains[J]. Electronic Journal of Emerging Infectious Diseases, 2025, 10(3): 1-5.

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深圳市某花鸟市场鹦鹉热衣原体采样调查与分离株基因组学研究

Surveillance of Chlamydia psittaci in a bird market in Shenzhen and genomic characterization of isolated strains

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