Surveillance of Chlamydia psittaci in a bird market in Shenzhen and genomic characterization of isolated strains

doi:10.19871/j.cnki.xfcrbzz.2025.03.001

Abstract

Abstract: Objective To screen for the presence of Chlamydia psittaci (Cps) in parrots from bird and flower markets in Shenzhen, and to conduct a comprehensive analysis of the positive samples, providing a scientific basis for epidemiological research, disease prevention and control, and public health management. Method In May 2023 and June 2024, two rounds of investigation and sampling were conducted at a parrot retail stall in a wholesale agricultural market in Shenzhen. The collected samples were screened for Cps, and Cps-positive samples were subjected to isolation through cell culture. Whole-genome sequencing was performed on the isolated Cps strains, followed by genomic analysis to explore genotype distribution and evolutionary relationships. Result A total of 110 samples were collected, of which 38 tested positive for Cps, with a positivity rate of 34.5% (38/110). One Cps strain was successfully isolated from the intestinal mucosa of a Cps-positive parrot. Whole-genome sequencing yielded the complete genome of the isolate, which was identified as genotype A. Phylogenetic analysis revealed that this strain is genetically closest to reference strains derived from parrots, suggesting potential host adaptation. Conclusion A relatively high detection rate of Cps was observed in samples collected from the parrot retail stall in a wholesale agricultural market in Shenzhen. It is recommended to strengthen hygiene management in bird and flower markets, especially at parrot stalls, and to enhance ongoing surveillance of Cps.

Key words: Chlamydia psittaci, Genomics, Cell isolation, Carrier status investigation and analysis

CLC Number:

R47.55

Cui Wenkai, Wu Shuang, Chen Qiongcheng, Lei Xinyue, Yuan Li, Cai Jianguang, Shi Xiaolu. Surveillance of Chlamydia psittaci in a bird market in Shenzhen and genomic characterization of isolated strains[J]. Electronic Journal of Emerging Infectious Diseases, 2025, 10(3): 1-5.

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