MALDI-TOF MS technique was used to analyze the bacterial community characteristics of mycobacterium contamination in liquid culture tubes

doi:10.19871/j.cnki.xfcrbzz.2024.02.002

Abstract

Abstract: Objective To understand the characteristics of contaminated bacteria in liquid culture of mycobacterium, analyze the causes of contamination, and find ways to reduce pollution. Method Collect sputum and bronchoalveolar lavage fluid samples from the Guangxi Zhuang Autonomous Region Chest Hospital between May 10, 2023,and August 25, 2023 for liquid culture of Mycobacterium.Using MALDI-TOF MS technology to identify contaminated bacteria produced by liquid culture of Mycobacterium, classify the identified strains statistically, and analyze the microbial community characteristics of contaminated bacteria. Result A total of 864 Mycobacteria were cultured in 6090 respiratory tract samples, the positive rate was 14.19% (864/6090), and the contamination rate was 1.17% (71/6090). The main polluting bacteria were Bacillus and Aspergillus, accounting for 31.08%(23/74) and 24.32%(18/74), respectively. Among the 1429 cases of lavage, 279 cases of mycobacterium were cultured, the positive rate was 19.52% (279/1429), and the pollution was 13 cases, contamination rate was 0.91% (13/1429). In 4661 sputum samples, 585 strains of mycobacterium were cultured. The culture positive rate was 12.55% (585/4661) and the pollution was 58 cases,contamination rate was 1.24% (58/4661). The positive rate between the two specimens exhibited a statistically significant difference (χ²= 43.68, P< 0.05), while no statistically significant difference was observed in the contamination rate (χ²= 1.06, P>0.05). Conclusion The contamination microbial community generated during liquid culture of Mycobacterium is complex, mainly composed of Bacillus and Aspergillus. Therefore, it is necessary to standardize the collection and transportation of specimens, and adopt effective pre-treatment methods to reduce the contamination rate of liquid culture.

Key words: Polluting bacteria, Mycobacterium, Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

CLC Number:

Lan Jihui, Tang Liusheng, Huang Kui, Liu Aimei, Dong Jie. MALDI-TOF MS technique was used to analyze the bacterial community characteristics of mycobacterium contamination in liquid culture tubes[J]. Electronic Journal of Emerging Infectious Diseases, 2024, 9(2): 7-10.

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