Distribution of pathogenic bacteria in patients with ventilator-associated pneumonia and detection of serum miR-101-3p and STC1

doi:10.19871/j.cnki.xfcrbzz.2024.02.005

Abstract

Abstract: Objective To investigate the distribution of pathogenic bacteria in patients with ventilator-associated pneumonia (VAP) and the clinical significance of detecting the expression levels of serum microRNA-101-3p (miR-101-3p) and stanniocalcin 1 (STC1). Method From January 2020 to April 2023, 121 patients who underwent mechanical ventilation treatment and developed VAP in our hospital were collected as the VAP group, 87 patients who did not develop VAP were regarded as the non VAP group, and 83 healthy individuals were collected as the control group. Lower respiratory secretions were collected from patients in the VAP group for identification of pathogenic bacteria; Serum STC1, procalcitonin (PCT), C-reactive protein (CRP), tumor necrosis factor-α (TNF-α), and serum miR-101-3p levels were measured in all study subjects. Pearson method was applied to analyze the correlation between serum miR-101-3p, STC1, and inflammatory factors PCT, CRP, and TNF-α; receiver operating characteristic curve (ROC curve) was applied to analyze the diagnostic value of serum miR-101-3p and STC1 for VAP. Result A total of 218 strains of pathogenic bacteria were detected and isolated from 121 VAP patients, of which 124 (56.88%) were Gram negative bacteria, 86 strains (39.45%) were Gram positive bacteria, and 8 strains (3.67%) were fungi; the serum levels of miR-101-3p, PCT, CRP, and TNF-α in the VAP group were obviously higher than those in the non VAP group and control group (P< 0.05), while the level of STC1 was obviously lower than that in the non VAP group and control group (P<0.05); the serum miR-101-3p level in VAP patients were positively correlated with PCT, CRP, and TNF-α(P<0.05), while STC1 level were negatively correlated with PCT, CRP, and TNF-α (P<0.05); ROC curve showed that the area under the curve (AUC) of serum miR-101-3p and STC1 alone and in combination for diagnosis of VAP patients were 0.766, 0.750, and 0.841, respectively, the combined diagnostic value was higher than that of the two alone (Z combination-miR-101-3p=2.275, P=0.023, Z combination-STC1=2.856, P=0.004). Conclusion The patients with VAP were predominantly infected with Gram negative bacteria, and the serum miR-101-3p is highly expressed, while STC1 is low expressed. The combination of serum miR-101-3p and STC1 has certain diagnostic value for the occurrence of VAP.

Key words: Ventilator-associated pneumonia, Distribution of pathogenic bacteria, Micro RNA-101-3p, Stanniocalcin 1

CLC Number:

R183.3

Yu Chao, Shao Qiang, Chen Li, Huo Qianwen. Distribution of pathogenic bacteria in patients with ventilator-associated pneumonia and detection of serum miR-101-3p and STC1[J]. Electronic Journal of Emerging Infectious Diseases, 2024, 9(2): 24-28.

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