Sequence analysis of pol gene of HIV-1 strain in Dongguan

doi:10.19871/j.cnki.xfcrbzz.2023.06.002

Abstract

Abstract: Objective To investigate the epidemiological characteristics of human immunodeficiency virus type 1 (HIV-1) and the subtypes distribution among HIV-1-infected individuals in Dongguan. Method From January 2019 to January 2021, 284 HIV-1 infected patients who had received antiviral treatment in Dongguan were selected, and was collected their epidemiological information. The viral RNA of HIV-1 was extracted from plasma. The pol gene was amplified by nested polymerase chain reaction. and gene sequence analysis was performed using biological software such as LaserGene, HIV BLAST, and MEGA 6.0. Result A total of 284 samples were collected in this study and finally successfully obtained 269 HIV-1 pol gene sequences. Among the 269 samples, 86.2% (232/269) were male, 61.3% (165/269) were younger than 40 years old, 52.4% (141/269) were unmarried, and 49.4% (133/269) were heterosexual and 47.2% (127/269) were homosexual. Of the 269 pol gene sequences, eight genetic subtypes were identified: 131 (48.7%) CRF07_BC, 59 (21.9%) CRF01_AE, 38 (14.1%) CRF55_01B, 19 (7.1%) subtype B, 12 (4.5%) CRF08_BC, and 10 (3.7%) other subtypes. Among 133 patients with heterosexual transmission, the predominant subtypes were 59 (44.4%) CRF07_BC, 32 (24.1%) CRF01_AE, and 15 (11.3%) CRF55_01B, and among 127 patients with homosexual transmission, the predominant subtypes were 65 (51.2%) CRF07_BC, 26 (20.5%) CRF01_AE, and 23 (18.1%) CRF55_01B. Differences in gender and marital status composition among individuals infected with different subtypes were statistically significant (P<0.05), while no statistically significant differences were observed in age and mode of infection composition. Conclusion The distribution of HIV-1 subtypes in Dongguan is complex and diverse, with CRF07_BC, CRF01_AE, and CRF55_01B being predominant. Furthermore, significant differences exist among HIV-infected individuals of different genders and marital statuses.

Key words: Human immunodeficiency virus typeⅠ, Sequence analysis, Subtype distribution

CLC Number:

R512.91

He Songmei, Liang Jinhu, Zhong Qingyang, Cao Huanhuan, Yin Juzhen, Wang Fuxiang. Sequence analysis of pol gene of HIV-1 strain in Dongguan[J]. Electronic Journal of Emerging Infectious Diseases, 2023, 8(6): 9-13.

References

[1] SEPKOWITZ KA.Opportunistic Infections in Patients with and Patients without Acquired Immunodeficiency Syndrome[J]. Clin Infect Dis, 2002, 34(8): 1098-1107.
[2] FINZI D, HERMANKOVA M, PIERSON T, et al.Identification of a reservoir for HIV-1 in patients on highly active antiretroviral therapy[J]. Science, 1997, 278(5341): 1295-1300.
[3] WONG JK, HEZAREH M, GUNTHARD HF, et al.Recovery of replication-competent HIV despite prolonged suppression of plasma viremia[J]. Science, 1997, 278(5341): 1291-1295.
[4] GAO D, ZOU Z, DONG B, et al.Secular trends in HIV/AIDS mortality in China from 1990 to 2016: gender disparities[J]. PLoS One, 2019, 14(7): e0219689.
[5] SONG H, GIORGI EE, GANUSOV VV, et al.Tracking HIV-1 recombination to resolve its contribution to HIV-1 evolution in natural infection[J]. Nat Commun, 2018, 9(1): 1928.
[6] ZHOU PP, YU G, KUANG YQ, et al.Rapid and complicated HIV genotype expansion among high-risk groups in Guangdong Province, China[J]. BMC Infect Dis, 2019, 19(1): 185.
[7] 于鑫, 赵鹏宇, 岳超, 等. 哈尔滨市艾滋病患者抗病毒治疗耐药情况及影响因素分析[J]. 中华疾病控制杂志, 2016, 20(12): 1194-1198.
[8] BAIRD HA, GAO Y, GALETTO R, et al.Influence of sequence identity and unique breakpoints on the frequency of intersubtype HIV-1 recombination[J]. Retrovirology, 2006, 3(1): 91.
[9] 苏迎盈, 刘慧鑫, 吴静, 等. 中国HIV-1基因亚型分布及流行趋势[J]. 中华流行病学杂志, 2014, 10: 1164-1168.
[10] 杨普贤, 汤赵荣, 刘伟元, 等. 2002~2019年东莞市大岭山镇艾滋病疫情数据统计及流行病学特征分析[J]. 中国实用医药, 2021, 16(3): 134-136.
[11] 刘绍青, 覃晓丹, 徐永芳, 等. 2016—2021年南宁市≥50岁人群HIV/AIDS病例流行特征分析[J]. 医学动物防制, 2023, 39(11): 1077-1079,1084.
[12] 成慧, 刘登来, 刘家虹, 等. 江西省某县嫖客人群艾滋病流行病学分析[J]. 中国公共卫生, 2018, 34(9): 1295-1298.
[13] 陈献军, 陈允刚, 梁英芳, 等. 参与式健康教育方法在农村艾滋病疫情严重地区的应用[J]. 应用预防医学, 2017, 23(3): 234-236.
[14] 伦玉华, 罗皓, 方晓君, 等. 东莞市HIV-1流行株pol基因序列特征和亚型分析[J]. 中华疾病控制杂志, 2018, 22(12): 1297-1299.
[15] LI Y, HAN Y, XIE J, et al.CRF01_AE subtype is associated with X4 tropism and fast HIV progression in Chinese patients infected through sexual transmission[J]. AIDS, 2014, 28(4): 521-530.
[16] GE Z, FENG Y, LI K, et al.CRF01_AE and CRF01_AE Cluster 4 Are Associated With Poor Immune Recovery in Chinese Patients Under Combination Antiretroviral Therapy[J]. Clin Infect Dis, 2021, 72(10): 1799-1809.
[17] ZAI J, LIU H, LU Z, et al.Tracing the transmission dynamics of HIV-1 CRF55_01B[J]. Sci Rep, 2020, 10(1): 5098.
[18] 方晓君, 罗皓, 伦玉华, 等. 东莞市HIV-1主要毒株基因分型及其对耐药影响的研究[J]. 华南预防医学, 2020, 46(3): 227-230,234.
[19] 钟平. HIV 分子流行病学研究和实践进展[J/CD]. 新发传染病电子杂志, 2019, 4(3): 137-144.
[20] ZHANG D, ZHENG C, LI H, et al.Molecular surveillance of HIV-1 newly diagnosed infections in Shenzhen, China from 2011 to 2018[J]. J Infect, 2021, 83(1): 76-83.
[21] 邱钰婷, 李健健, 刘家法, 等. 云南省昆明市HIV-1流行株pol基因序列特征分析[J]. 中华疾病控制杂志, 2022, 26(3): 309-314.
[22] 洪亮, 陶静, 李佳洁, 等. 上海市虹口区2016 年新报告HIV-1 感染者基因亚型和耐药监测情况[J]. 上海预防医学, 2022, 34(9): 860-864,878.
[23] 宋潇, 李婷, 江云飞, 等. 黑龙江省588例新报告HIV-1感染者病毒基因特征及耐药分析[J]. 中国艾滋病性病, 2022, 28(7): 781-785.
[24] LI QH, WANG JY, LIU SY, et al.Young MSM changed temporal HIV-1 epidemic pattern in Heilongjiang Province, China[J]. Front Microbiol, 2022, 13: 1028383.
[25] 乔瑞娟, 张林才, 冯毅, 等. 甘肃省部分地区2020-2021年新报告HIV/AIDS患者治前耐药及分子传播网络特征分析[J]. 中国艾滋病性病, 2023, 29(08): 860-865.