金银花对轻型新型冠状病毒肺炎治疗的药理价值

doi:10.19871/j.cnki.xfcrbzz.2021.03.004

新发传染病电子杂志 ›› 2021, Vol. 6 ›› Issue (3): 181-188.doi: 10.19871/j.cnki.xfcrbzz.2021.03.004

金银花对轻型新型冠状病毒肺炎治疗的药理价值

郭津含¹, 陈建林², 葛岚岚^3,4, 万浩强³, 杨天庆¹, 缪雨阳^3,4, 肖凌云^3,4, 凌云志³, 曾小斌^3,4

1.暨南大学第二临床学院深圳市人民医院龙华分院超声科,广东深圳 518120;
2.暨南大学第二临床学院深圳市人民医院龙华分院普外科,广东深圳 518120;
3.暨南大学第二临床学院深圳市人民医院龙华分院中心实验室,广东深圳 518120;
4.暨南大学第二临床学院深圳市人民医院感染内科,广东深圳 518120

收稿日期:2021-07-10 出版日期:2021-08-31 发布日期:2021-10-11
通讯作者: 凌云志,Email: lyz@algaemedical.com;曾小斌,Email: zeng.xiaobin@szhospital.com
基金资助:
1.国家自然科学基金（81903760,81903914）; 2.中国博士后科学基金（2018M633290）; 3.深圳市创新委员会基础研究项目（JCYJ20180228164437735）; 4.广东省医学科研基金项目（A2019333）

Pharmacological value of Lonicera japonica flos on the treatment of mild COVID-19 patient

Guo Jinhan¹, Chen Jianlin², Ge Lanlan^3,4, Wan Haoqiang³, Yang Tianqing¹, Miao Yuyang^3,4, Xiao Lingyun^3,4, Ling Yunzhi^3,4, Zeng Xiaobin^3,4

1. Department of Ultrasound, Longhua Branch, Shenzhen People's Hospital, the Second Clinical Medical College of Jinan University, Guangdong Shenzhen 518120, China;
2. Department of General Surgury, Longhua Branch, Shenzhen People's Hospital, the Second Clinical Medical College of Jinan University, Guangdong Shenzhen 518120, China;
3. Center Laboratory, Longhua Branch, Shenzhen People's Hospital, the Second Clinical Medical College of Jinan University, Guangdong Shenzhen 518120, China;
4. Department of Infectious Diseases, Shenzhen People's Hospital, the Second Clinical Medical College of Jinan University, Guangdong Shenzhen 518120, China

Received:2021-07-10 Online:2021-08-31 Published:2021-10-11

摘要/Abstract

摘要： 目的探讨金银花提取物对轻度新型冠状病毒肺炎(COVID-19)的潜在治疗药理价值,为轻型COVID-19提供中医药防治新思路。方法利用中药系统药理学分析平台（TCMSP）筛选出金银花活性成分及作用靶点。SwissADME和pkCSM数据库预测成分药物代谢动力学特性。基因表达综合（GEO）数据库收集轻型COVID-19转录测序组数据。数据质控,DEGs鉴定及富集分析由R工具包实现。STRING和Cytoscape构建蛋白相互作用网络,利用cytoHubba插件筛选核心DEGs。运用PyMOL软件进行分子对接。结果金银花7种活性成分具有较好的口服吸收利用度和类药性特征。与轻型COVID-19相关的靶点3个：COL20A1、一氧化氮合酶2（NOS2）、双氧化酶2（DUOX2）。其共同显著富集通路5条,包括过氧化物代谢,活性氧生物合成及代谢,血红素、四吡咯结合等途径。结论金银花治疗轻型COVID-19具有多靶点多通路作用的特点。其可能作用机制是通过NOS2、DUOX2表达调控轻型COVID-19发展过程中的活性氧与血红素相关过程对这类患者进行干预。

关键词: 金银花, 活性氧, 血红素加氧酶-1, 一氧化氮合酶2, 双氧化酶2, 轻度新型冠状病毒肺炎

Abstract: Objective The purpose of this study was to investigate the potential pharmacological value of Lonicera japonica flos extract in treating mild COVID-19, and to provide a new idea of TCM prevention and treatment for mild COVID-19. Methods TCMSP database was used to screen out the active components and action targets of honeysuckle. SwissADME and pkCSM databases were used to predict pharmacokinetics of constituents.The GEO database collected transcriptional sequencing data for mild COVID-19. Data quality control, DEGs identification and enrichment analysis were realized by R tool. STRING and Cytoscape constructed protein interaction networks and screened core DEGs using cytoHubba. Molecular docking was performed using PyMOL software. Results The 7 active ingredients of Honeysuckle had good oral absorption and utilization and drug-like characteristics.There were three targets associated with mild COVID-19: COL20A1, NOS2, and DUOX2. There were 5 common enrichment pathways, including peroxides metabolism, reactive oxygen biosynthesis and metabolism, heme, tetrapyrrole binding and so on. Conclusion Honeysuckle had the characteristics of multi-target and multi-pathway action in the treatment of mild COVID-19. The possible mechanism of action was to intervene in mild COVID-19 by regulating reactive oxygen species and heme related processes during the development of mild COVID-19 through NOS2 and DUOX2 expression.

Key words: Honeysuckle, Reactive oxygen species, Heme oxygenase 1, Nitric oxide synthase 2, Dual oxidase 2, Mild corona virus disease 2019

郭津含, 陈建林, 葛岚岚, 万浩强, 杨天庆, 缪雨阳, 肖凌云, 凌云志, 曾小斌. 金银花对轻型新型冠状病毒肺炎治疗的药理价值[J]. 新发传染病电子杂志, 2021, 6(3): 181-188.

Guo Jinhan, Chen Jianlin, Ge Lanlan, Wan Haoqiang, Yang Tianqing, Miao Yuyang, Xiao Lingyun, Ling Yunzhi, Zeng Xiaobin. Pharmacological value of Lonicera japonica flos on the treatment of mild COVID-19 patient[J]. Electronic Journal of Emerging Infectious Diseases, 2021, 6(3): 181-188.

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金银花对轻型新型冠状病毒肺炎治疗的药理价值

Pharmacological value of Lonicera japonica flos on the treatment of mild COVID-19 patient

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